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The considerations agreed by other authors Carpinella et al. Other studies Bagavan et al. In particular, Abdel-Ghaffar et al. The hexane flower bud extract of S.
These studies underline that natural products may provide good antilice activity and can offer a valid alternative to conventional insecticides. The results obtained from this study presented a promising scenario for using combinations of tea tree oil and nerolidol as effective alternative for treating pediculosis. Essential oils are potential natural products for lice control, promoting selective effects against resistant insects, and may prevent the rapid development of resistance, in particular in developed countries Mumcuoglu et al.
In Italy, very few studies were carried out Sidoti et al. Moreover, studies of pediculicidal and ovicidal effectiveness of new products should be performed with lice collected in the country to obtain real efficacious treatments. Our analysis provides efficacy against lice and their eggs encouraging for novel therapy schemes including natural compounds as alternative approaches to cure P.
Moreover, in this study, we avoid misidentifying mortality following stringent criteria for the definition of mortality of lice and louse eggs. As suggested by Yang et al. The essential oils are complex mixtures of many different components with various degrees of lipophilicity, relative hydrophilicity, and volatility at room temperature. The terpenoid components of essential oils as tea tree oil can exert their action in a mechanical way. Because of the low molecular weight, such compounds can pass through the cuticle of louse up to the trachea causing the death of head lice by suffocation.
The efficacy of essential oils can be attributable to the combined effect of direct deposition of essential oils on cuticle together with an indirect effect via adsorption of the vapors. Then, to potentiate this effect, it will be useful to apply oils, cover hair with a shower cap, and let stand overnight with the aim to suffocate the remaining live lice nymphs.
The results of this study offer new potential application of natural compounds. Education of parents and teachers about louse biology, epidemiology, control, and an effective prevention is of paramount importance to manage lice infestation. In fact, the increasing knowledge of the pediculosis, overcoming prejudice, should favor a rapid alert of health service, providing suitable treatment.
Nymph showing persistent internal movements of gut Video 1 and dead adult louse and nymph showing persistent movements of limbs Video 2 after 30 min of treatment at D4 condition tea tree oil 0.
The authors thank Mrs Anamaria Pereira for her kind contribution. This Research was supported by Union Health S.
Giovanni Teatino, CH Italy. This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author s and the source are credited.
Di Campli and S. Di Bartolomeo contributed equally to this work. Parasitology Research. Parasitol Res. Published online Jul Author information Article notes Copyright and License information Disclaimer. Corresponding author. Received Mar 19; Accepted Jul Abstract Head lice infestation is an emerging social problem in undeveloped and developed countries.
Electronic supplementary material The online version of this article doi Introduction Head lice infestation is caused by Pediculus humanus capitis De Geer, belonging to the family Pediculocidae, permanent and strictly obligatory ectoparasite species-specific: it completes the entire life cycle on the scalp of man.
Materials and methods Head lice and louse eggs collection Adults, nymphs, and eggs of P. Open in a separate window. Products Tea tree oil oil of melaleucaterpinenol type, Fig. Bioassay Pediculicidal activity For testing pediculicidal activity, the baskets containing adults and nymphs detected together of lice, distributed in Petri dishes lined with Wathman no.
Ovicidal activity For testing ovicidal activity, all the viable selected louse eggs, distributed in Petri dishes with Wathman no. Results The results obtained by treating 69 head lice, adults and nymphs, with tea tree oil A , nerolid B and their combinations in ratios C and D at different concentrations are shown in Fig. Discussions The present study aimed to evaluate the efficacy of two natural products, tea tree oil and nerolidol, at various concentrations, used alone or combined in ratios and , against lice and their eggs.
Electronic supplementary material Nymph showing persistent internal movements of gut Video 1 and dead adult louse and nymph showing persistent movements of limbs Video 2 after 30 min of treatment at D4 condition tea tree oil 0. Acknowledgments The authors thank Mrs Anamaria Pereira for her kind contribution. Giovanni Teatino, CH Italy Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author s and the source are credited.
Footnotes E. Efficacy of neem seed extract shampoo on head lice of naturally infected humans in Egypt. Efficacy of a single treatment of head lice with a neem seed extract: an in vivo and in vitro study on nits and motile stages. Antileishmanial activity of the terpene nerolidol. Antimicrob Agents Chemother. Contact and fumigant toxicity of hexane flower bud extract of Syzygium aromaticum and its compounds against Pediculus humanus capitis Phthiraptera: Pediculidae Parasitol Res.
BMC Dermatol. Sensitization of Staphylococcus aureus and Escherichia coli to antibiotics by the sesquiterpenoids nerolidol, farnesol, bisabolol, and apritone. Human lice and their control. Annu Rev Entomol. BMC Pharmacol. BMC Res Notes. Do head lice spread in swimming pools? Int J Dermatol. A comparison of botanical and synthetic substances commonly used to prevent head lice Pediculus humanus var. In vitro pediculicidal and ovicidal activity of an extract and oil from fruits of Melia azedarach L.
J Am Acad Dermatol. Melaleuca alternifolia tea tree oil: a review of antimicrobial and other medicinal properties. Clin Microbiol Rev. Head lice Pediculus humanus capitis. Public information. Fact sheet. Scabies and pediculosis. Laboratory evaluation of aromatic essential oils from thirteen plant species as candidate repellents against Leptrombidium chiggers Acari: Trombiculidae , the vector of scrub typhus.
Exp Appl Acarol. Pediculosis and scabies. Am Fam Physician. Cancer chemoprevention and therapy by monoterpenes. Environ Health Perspect. A new shampoo based on neem Azadirachta indica is highly effective against head lice in vitro.
In vitro efficacy of over-the-counter botanical pediculicides against the head louse Pediculus humanus var capitis based on a stringent standard for mortality assessment. Med Vet Entomol. Susceptibility of head lice Pediculus humanus capitis to pediculicides in Australia. The antibacterial effects of terpene alcohols on Staphylococcus aureus and their mode of action. Fragrance material review on nerolidol isomer unspecified Food Chem Toxicol.
Molecular analysis of kdr -like resistance in Permethrin-resistant strains of head lice, Pediculus capitis. Pestic Biochem Physiol. Antifungal effect of eugenol and nerolidol against Microsporum gypseum in a guinea pig model. Biol Pharm Bull. Pediculosis capitis. J Pediatr Health Care. Antimalarial use of volatile oil from leaves of Virola surinamensis Rol. J Ethnopharmacol. Are commercially available essential oils from Australian native plants repellent to mosquitoes.
J Am Mosq Contr Assoc. Ovicidal effects of a neem seed extract preparation on eggs of body and head lice. Embryonic development of human lice: reaaring conditions and susceptibility to spinosad. Prevention and treatment of head lice in children. Paediatr Drugs. With health-related knowledge doubling in months, AI will become a mandatory survival kit. Yet, it still will see things that human eye might miss.
Or making correlations that are simply too difficult anyway else. Yes, it will be both defensive for professionals and offensive to diseases, if used in the right way, as every tool. Digital will influence behaviors, create communities, and redefine the patient-professional interaction. Patients are individuals, parents, children, workers, citizens, consumers, with ever-growing expectations on what and how can be done through a smartphone. Space and satellite technology will provide the communication background for all above, from remote surgery to distributed expert network, etc.
But Space will also bring additional data coming from macroscopic data gathering, earth observation, context-related data and gravity-less phenomenon analysis. Can the lab of the future stay immune from all above? Hard to believe. While exact predictions are useless, some trends are clearly visible and point to the raise of a next-generation ubiquitous lab. The complete blood count CBC is one of the most requested tests, routinely performed in the central laboratory LAB by large haematological analysers, useful to diagnose many diseases and manage urgent clinical decisions such as transfusion or administration of chemotherapy and antibiotics.
Hb remains the most common POCT in haematology, essential to exclude anaemia. There are two types of technology: small benchtop analysers and portable devices. The latter, some of which use disposable cartridges, do not require start-up procedures, maintenance and calibrations. The latest generation of portable devices combines advanced digital technology with innovative technologies of viscoelastic focusing and microfluidics and techniques, such as digital microscopy and computer vision, using near infrared spectroscopy and the absorption of light at multiple wavelengths, obtaining CBC results unthinkable until a few years ago.
Poor finger prick technique can provide misleading results, it was proved that capillary samples significantly underestimate PLTs overestimating Hb and WBCs, but differences have not clinical relevance when the samples are collected according to standardized procedures. POCT devices can not differentiate normal cells from pathological ones e.
As recommended by the guidelines, due to the inherent risk of preanalytical errors and the standard risk of error during analytical and postanalytical phases, threshold values must be established to repeat CBC in a LAB.
Literature suggests that POCTs are not yet the ideal tools to perform CBC for diagnostic purposes, but they are useful in urgent situations such as rapid monitoring of some parameters e. Further studies are needed to confirm the promising results of POCTs and evaluate their performance even at low ranges and in pathological conditions.
Rampoldi E. Carraro P. Biochim Clin ; Briggs C. Where are we at with point-of-care testing in haematology?. BJH ; — Mooney C. Point of care testing in general haematology. Machine learning algorithms have proven to be very effective in predicting the behavior of phenomena represented in biomedical data. The most commonly used machine learning algorithms, such as artificial neural networks, produce so-called “black box” results, namely: a complex set of mathematical equations that cannot be interpreted by people who do not have in-depth mathematical skills;.
When applying machine learning to data such as images, black box algorithms are not a problem, since the value of the model lies in its accuracy in detecting the presence of certain patterns, attributable, for example, to the presence of a tumor. A specific ML technique, the Rulex “rule generation method”, builds models described by a set of intelligible rules, thus allowing the extraction of important knowledge regarding the variables included in the analysis and their relationships with the outcomes of the phenomenon analyzed.
Introduction: Point-of-care testing POCT is laboratory testing conducted close to the site of patient care. POCT is growing in popularity with manufacturers offering a wide menu of tests and devices where the operator can obtain a rapid test result with the potential to initiate faster patient care decisions. But POCT is not fool-proof, and any test can and will fail if operated under the wrong conditions.
Methods: Risk management is a process where laboratories can assess their weaknesses, implement a control plan to detect and prevent erroneous results, and monitor the effectiveness of their plans. Results: The Clinical and Laboratory Standards Institute CLSI EPA: Quality Control Based on Risk Management provides guidance based on risk management for laboratories to develop quality control plans tailored to the particular combination of measuring system, laboratory setting, and clinical application of the test.
Discussion: This presentation will describe how laboratories can partner with manufacturers to conduct risk assessments and implement quality control plans in their laboratory and at the point-of-care.
The advantages of utilizing a risk management approach to controlling laboratory errors will be emphasized along with the efficiencies gained from conducting a risk assessment and implementing a quality control plan. A revision of CLSI EPA is currently being drafted, and this presentation will preview a few of the updates that can be expected in the next version of the guidance document. Evidence based laboratory medicine EBLM focuses on the use of diagnostic tests to improve patient outcomes.
POC are tests conducted near the site of patient care, outside of the laboratory, usually performed by patients or clinical personnel not trained in laboratory medicine. POCT require small sample volumes, minimize pre-analytical errors, and reduce alterations of labile analytes. However, when used appropriately, could improve the patients outcomes by providing faster results and earlier therapeutic strategies 2.
Instead, its over or incorrected use could leads a patient risk and potential increase of healthcare costs. We assessed, through a systematic review of the recent scientific literature, the accuracy of the POCT on troponin, procalcitonin, C-reactive protein, parathyroid hormone, INR and d-dimer, and evaluate the impact of faster results on patient management. Instead, studies on CRP claimed a significant reduction of antibiotic prescription. Several authors evaluated troponin and INR reporting faster decision-making without any improvement in clinical outcome.
Faster results are often translated in better outcomes, without evidence to support this conclusion. So, it is important that the POCT practice is evidence-based looking for evidence of whether POCT confers any advantage in clinical decision making in different scenarios. In some settings, such as rural environment, a rapid availability of cardiac troponins or other analytes can help clinicians to rule out or rule in disease, without transfer patient in other center, avoiding unnecessary costs 3.
Likewise, in Emergency Department, availability of more rapid results with POCT help clinicians to refer patients, but does not always translate into shorter stays 4. The satisfactory analytical performance, together with an excellent practicability, suggest that the POCT represents an important technological advance in patient care, but, the lack of evidence about the patients outcome invite healthcare workers to use it with judgement.
Price CP. Point of care testing. BMJ ; — Ann Clin Biochem. Arch Pathol Lab Med. Alter DN. Arterial and venous blood gas analysis reveals oxygenation and acid-base status of the body. Hemoximetry is recommended to determine the impact of dyshemoglobins on oxygenation.
Some calculated values may be in error, e. Moreover, the presence of high concentration of fetal hemoglobin may also be a problem if blood gas analyzer does not detect it, as instrument assumes hemoglobin to be of the adult type, and therefore the calculated blood gas oxygen saturation values are underestimated. In critically ill patients many other analytes have been used to estimate the severity of disease and try to prognosticate morbidity and mortality.
No measurements can encompass the complexity of a disorder, but lactic acid can approach that goal 3 Indeed lactic acidosis is the most frequent metabolic acidosis and many causes are reported for lactate increase, not only hypoxia: the higher the lactate concentration, the worse the outcome. The initial values have a prognostic significance, but serial measurements are more valuable for prognosis. Conductivity-based Hematocrit Ht estimations have limitations. Abnormal protein concentration will change plasma conductivity.
Low protein concentration, resulting from dilution of blood with protein-free electrolyte solution during surgery, will result in erroneously low Ht value. In any situation, to correctly interpret BGA results history should be always considered: reasons for presentation, information concerning events, environment, trauma, medications, poisons, toxins and an accurate physical examination should be carefully collected. Acute respiratory distress syndrone: the berlin definition, Ranieri MV et al.
Conductivity-based Hematocrit measurement during cardiopulmonary bypass. Steinfelder-Visccher J et al. The knowledge that has been garnered so far on severe acute respiratory syndrome coronavirus 2 SARS-CoV-2 infection is that humoral immunity encompasses the generation of immunoglobulins of most classes against surface viral antigens, which mostly involve the spike protein, the nucleocapsid protein, but also envelope and membrane proteins.
Since the spike protein is the anchor that the virus uses for penetrating the host cells through biding with its natural host cells receptors, it can be assumed that antibodies binding to spike protein of SARS-CoV-2, and especially to its receptor binding domain, would retain stronger neutralizing potency against the virus.
Serological testing has been conventionally defined as a diagnostic procedure used for detecting an immune response against an infectious agent. The diagnostic sensitivity stratified according to the assay methodology is highly variable. Additionally important drawbacks of rapid serological tests include the facts that the information provided by the companies is concerning because often lacks details, its quality is considerably variegated among different devices, several claims are vague, there is a lack of transparency along with the fact that human aspects are not been adequately addressed for purpose of alleviating the risk of inappropriately using the device.
The risk of misinterpreting tests results by patients when rapid kits are used for self-diagnosis is another aspect that must be considered.
This was mostly due to objective difficulties encountered by the patients in reading and interpreting the results of the strips. Important considerations for implementing point-of-care serology testing thus include i usage of well-validated tests, evaluated against a gold standard; ii performance characteristics – thus encompassing sensitivity, specificity, positive and negative predictive values or cross-reaction with other coronaviruses – shall be tested using serum samples collected from patients infected with SARS-CoV-2, with other respiratory viruses including seasonal coronaviruses and also from healthy controls; iii adequate training of healthcare workers is needed iv and, finally, IV provisions must be in place, encompassing the capture of testing data for individual patient records and surveillance purposes, and the participation to external quality assessment schemes, to systematically monitor the quality of this type of testing.
At the heart of society 4. It is in these sectors that, lately, the greatest investments have been made in digital transformation aimed at exploiting -through data-all the new present and emerging technologies, from the Internet of Things IoT to Artificial Intelligence AI. The exploitation of Big Data, in fact, constitutes the starting point and the indispensable resource for the development of innovative and precision medicine, providing scientific, organizational and infrastructural support to promote research and accelerate preclinical and clinical studies.
However, this development, having increased the number of subjects holding health-related data, the speed of transmission of such data and the quantity of information electronically stored often not on national territory , has determined an exponential increase in the danger of data processing from the point of view of confidentiality and an increased possibility of damaging the dignity and fundamental freedoms of the individual.
This has led to an increased sensitivity of the European legislator and, subsequently, of the national legislator, towards the protection of such data and related protections. In addition to the General Data Protection Regulation, which has revolutionized the way of conceiving the data economy, it is, in fact, being evaluated by European institutions the first draft of the Artificial Intelligence Act, which will be the real springboard for the massive and regulated use of algorithms, especially in healthcare.
To be precise, this last mentioned regulation will only define the limits to the use of algorithmic systems already widely in use.
AIFA, through this guide, has described some case studies, showing some workflows that represent the regulations impacted depending on the type of system used and paying particular attention to the compliance related to the treatment of data and the related profiles of cybersecurity. Ad oggi sono operativi circa 72 Drive-Through-Difesa. I contributi, forniti da ciascuna Forza Armata, sono diretti e coordinati fin dalla prima ora dal Comando Operativo di vertice Interforze COI per mezzo di una Sala Operativa dedicata, composta da personale interforze.
In the last couple of decades, Laboratory Medicine has made giant steps forward in terms of innovative technology and has made major scientific breakthroughs in the medical field as a whole. Indeed, a plethora of both in vitro and in vivo assays and tests in biological fluids of the human hydrodynamic system are now available.
The importance, for clinical purposes, of novel metabolic processes and protein cross-talk mechanisms is being increasingly recognized. The increased survival period of sick, elderly people, plus the therapeutic aspects of precision medicine, in which the drugs selected resulted in a series of direct approaches to altered target molecules, have made it difficult to identify the most effective molecules to use as biomarkers in most of this population scenario. Therefore, it seems that Laboratory Medicine does not need to increase further value in the contribution to the care of fragile individuals, and in people affected by chronic degenerative diseases.
Notwithstanding all these premises, and the increase in Clinical Laboratory testing, which is, and will continue in the future to be an indispensable ally of medical care, the correct diagnosis of a single or of multiple diseases occurring in a single individual will benefit enormously from this Discipline, if some steps forward will be made.
I believe that the enormous amount of knowledge now accumulating in the field of Laboratory Medicine will revolutionize, not only the medical care of people, but, in the various areas of the medical scenario, also the field of Laboratory Medicine Science itself and the practice deriving from it.
In other words, we should all begin to be mindful of our state of health as early as about 20—25 years of age, when most auxological aspects have been reached, and sexual maturity completed.
Therefore, also healthy people should be monitored as well as patients, which should be one of the tenets of preventive medicine. Having said that, I must now say that chronological age is practically meaningless in calculating health status. This, of course, applies much more to multimorbidity; in fact, once identified them, measures can be made to eradicate or to delay the start or the progression of each illness, therefore determining a better state of health during the progression of chronological age.
The revolution I am talking about is to look at each individual when they are enjoying still normal health, as mentioned above. This approach may be considered too costly, but in effect it is much less costly than waiting for the appearance of an overt disease, which must then be treated for decades, frequently with very expensive drugs and tests laboratory and imaging. This will also support the joining of Preventive Medicine to effective Individualized Medicine. Salvatore F.
The shift of the paradigm between ageing and diseases. Clin Chem. Lab Med. During the second wave, the validation of SARS-CoV-2 antigen rapid diagnostic tests RDT has substantially changed testing strategies globally, since results were available within 30 min, reducing turnaround time and therefore exposure risk.
Recently, validated self-tests for SARS-CoV-2 based on the nasopharyngeal swab NPS or saliva have prompted for the empowerment of the general population in the fight against the spread of infectious.
Swabbing is a complex task requiring training and competency assessment, and thus they are performed by trained nurses or physicians. Recently, Tsang et al. The Authors concluded that saliva and nasal swabs are clinically acceptable alternatives to commonly used nasopharyngeal swabs.
Saliva is a matrix elective for self-collection, and molecular testing is reliable but require laboratory instrumentation to be performed. Indeed, antigen determination on salivary samples is still under debate [2]. Most of the errors occur in the preanalytical phase, with relatively few analytical and post-analytical errors.
Some issues arising during the pre-analytical phase of SARS-CoV-2 diagnostics regards: the time of swab, swabbing practice, sample handling and conservation and RNA extraction. NPS should be taken at the time of symptom onset when the highest viral load occurs in COVID, thus not the day immediately before and not too far from possible close contact with positive subjects.
Differently, sample preparation is a crucial factor for antigen testing, and centrifuged vs. In conclusion, self-testing could be of aid in the screening programs for reducing viral spread, but other alternatives are possible, such as self-collection of samples with analytical tests performed in clinical laboratories. These required the optimization of pre-analytical steps to reduce the impact on results.
Woloshin, S. Basso, D. In a broad and complex territory such as that of ULSS 6 Euganea, composed of five local health districts with a population of about Information is sent in real time to central laboratory using IT middleware, where data are validated and historicized.
Historicized data can be consulted and downloaded like other laboratory exams. Results: the project involved patients. From January 1st to June 30th the average frequency of determinations per patient was about 30 days, while the average number of determinations per patient was about 7,5.
This model simplifies management of both patients in IHC and followed by RMC, allowing easier access to the determination of PT-INR, with more constant therapy control and significant improvement of life quality. Ovarian cancer is the seventh most frequent malignancy in the female population worldwide and the leading cause of death among gynecological cancers. In Italy, about new cases were registered in The availability of a guide in the diagnostic paths is a requirement for general practitioners and specialists of other disciplines that arises from the need to guarantee the most appropriate, less demanding, more useful and less expensive diagnostic path.
The purpose of PDTAs is to increase the quality of perceived and effectively delivered care, improving outcomes and promoting patient safety through the use of the right resources needed.
The surgical approach plays a fundamental and essential role in the treatment program and the absent tumor residue has been defined as the only tumor residue associated with optimal survival curves and also the antiblastic chemotherapy, that always follows surgery in advanced ovarian cancer, gets better results after optimal surgery.
The first network of reference and dissemination of PDTAs is constituted by general medicine. Indeed, we cannot ignore sharing with the entire regional network of general practitioner, who will thus know who to send the patient with suspicion or already ascertained diagnosis of ovarian cancer, for an adequate diagnostic and therapeutic path, allowing feedback on the conditions and clinical-therapeutic pathways for individual patients.
In addition, information and reference relationships with voluntary associations and patient associations must be encouraged. They constitute a fundamental asset in the creation and development of information, health culture of primary and secondary prevention and support in home care or in assistance facilities even to the terminally ill.
Scarone , V. Dovere, C. Traverso, F. Background: The emergency caused by the Covid pandemic has forced the reformulation of the operating methods of the Health System, turning the spotlight on the need for greater interaction between hospital and territory.
This aspect is even more evident in patients needing for a more strict followup as those under antithrombotic therapy TAO , making clear the essential usefulness of digital tools and of new organizative models. Each patient was asked for an email address and signed consent to the computerized management of TAO therapy and forwarding of health documents.
Results: In order to monitor the expected results, the following indicators were evaluated: number of incoming phone calls; number of treatment plans issued for DOAC; time in range for patients in AVK; number of complications recorded in the period under review April —April Conclusions: The new organization, based on digital support of clinical monitoring, has received high appreciation from patients and consequently a greater compliance with the therapy protocol.
This management model has allowed an effective control both of the number and severity of adverse events, while the reduction of outpatient access has allowed to drastically reduce the infectious risk. In addition, e-mailing of reports and treatment plans allowed an optimization of human resources. Lorubbio 1 , F. Baldelli 1 , E. Bromo 5 , G. Caldarelli 4 , C. Donnini 1 , S. Fabbroni 1 , A. Fanelli 1 , M. Fantacci 2 , L. Gasbarri 1 , M. Mazzi 4 , A.
Periccioli 3 , P. Pugliano 4 , C. Rapini 2 , A. Rebuffat 3 , A. Sereni 1 , M. Sorini 1 , E. Tripodo 1 , A. Ognibene 1. The use of information and communication technologies ICT and E-Health can contribute to a reorganization by moving the focus of health care from the hospital to the territory. The aim of the present project is to evaluate of the complete blood count CBC test and the peripheral blood smear through digital images, shared and available to the team of the 12 TSE laboratories.
During implementation, the image analyzers and the staining adopted were compared, together with the quality indicators QI to support the new flows implemented between the peripheral laboratories and the Hub laboratory. The Passing-Bablok and Bland-Altman plot analysis performed for comparison of all elements of the blood count test, provided excellent results between the technologies and the different cell staining used data not shown.
The organization proposed in the project improves the analytical quality, harmonises the reporting and interpretation of analytical data, promotes uniform training, preparing for continuous professional comparison. Pelagalli 1,2 , A. Giovannelli 1,2 , C. Calabrese 1,2 , S.
Sarubbi 1,2 , M. Minieri 1,2 , M. Nuccetelli 1,2 , M. Pieri 1,2 , S. Bernardini 1,2. Sepsis is an infectious disease the etiology can be viral or bacterial with hight mortality, threatening human health. The aim of this study is to use leucocyte counts neutrophils and monocytes that are activated from pathogenic virus or bacteria and others morphological change with Mindray BCplus platform to diagnose sepsis early, quickly, conveniently and at low cost.
A total EDTA-k2 anticoagulant venous whole blood samples were collected: 70 control patients blood donors with a normal complete count blood and negative VES, and samples hospitalized at the emergency department with symptoms attributable to sepsis with PCT request. All data was divided in 4 groups: control group, group where sepsis cannot be confirmed, group with confirmed sepsis diagnosis and a group with sepsis from SARS-CoV-2 infection. The roc curves highlight acceptable sensitivity and specificity values for some haematological parameters and suggest a possible cut-off.
The BC plus can help the diagnosis of sepsis upon the admission to the emergency department using some morphological positional parameters. Pecoraro 1 , T. Pirotti 1 , T. Trenti 1 , M. Plebani 2. However, their immunological significance are currently undefined. There are many methods available for the detection of specific Abs whit suboptimal diagnostic accuracy and relatively high throughput capacity and less stringent specimen requirements compared to RNA-based assays.
We conduct a retrospective study analyzing with a big data analysis all samples collected between 11 March and 30 September All serum samples received at the laboratory were processed using qualitative and commercially available rapid lateral flow immunoassay tests for nCoV IgG and IgM. Positive results were confirmed using a chemiluminescent method. Subjects with a positive result were contacted from the Department of Public Health for further tests viral RNA research or subsequent serological tests for definitive diagnosis.
A total of 69, serological tests in 42, subjects and , oropharyngeal or nasopharyngeal swabs in 88, subjects were performed. Of the subjects with IgG negative and IgM positive results, a positivity was confirmed in 1. Subsequent serological testing confirmed IgG positivity in 8 subjects 1. Conversely, in subjects with IgG positive and IgM negative results, a positivity was confirmed in 7. Scaglione , C. Nardelli, M. Setaro, E.
The impairment of this pathway is a common characteristic of many tumors and it is frequently observed in breast and ovarian cancer. Samples were then pooled and sequenced on NextSeq Dx platform Illumina. Sequencing files were quality checked, analyzed and processed using our dedicated bioinformatics pipeline. In this workflow, LGAs profiles were calculated using whole genome sequencing data at low coverage 0.
The HRD score was then estimated by measuring the level of agreement in the segmentation profiles of each samples. Cosma 1 , A.
Padoan 1 , C. Di Chiara 2 , D. Rinaldi 1 , D. Gastaldi 3 , D. Basso 1 , C. Giaquinto 2 , M. Plebani 1.
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Likewise, in Emergency Department, availability of more rapid results with POCT help clinicians to refer patients, but does not always translate into shorter stays 4. Baldelli 1 , E. The total number of lice ranged from 2 to 10, for each product and for each concentration. Interestingly, dead nymphs were mainly characterized by the gut rupture with seepage into the thorax and limbs Fig. Boeddinghaus et al. Sarubbi 1,2 , M. BMC Res Notes. Introduction: Point-of-care testing POCT is laboratory testing conducted close to the site of patient care. The shift of the paradigm between ageing and diseases.❿
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